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Endotoxin Removal By Magnetic Separation Based Blood Purification

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  • Magnetic separationbased blood purification a

    Magnetic Separationbased Blood Purification A

    Recent studies report promising results regarding extracorporeal magnetic separation-based blood purification for the rapid and selective removal of disease-causing compounds from whole blood. High molecular weight compounds, bacteria and cells can be eliminated from blood within minutes, hence offe

  • US6194562B1 Endotoxin reduction in nucleic acid

    Us6194562b1 Endotoxin Reduction In Nucleic Acid

    The present method removes endotoxins from such solutions using silica-based materials, such as silica gel particles, magnetic silica particles, or diatomaceous earth. In a preferred aspect of the method of this invention, magnetic silica particles are used to isolate plasmid DNA from a lysate of gram-negative bacteria transformed with the ...

  • Binding of Functionalized Paramagnetic Nanoparticles to

    Binding Of Functionalized Paramagnetic Nanoparticles To

    Current technologies to endotoxin detection and removal for biopharmaceutical purification. ... of boronic acid-functionalized mesoporous silica-coated coreshell magnetic microspheres with large pores for endotoxin removal. Journal of Chromatography A 2019, 1602 , 91-99. ... Endotoxin Removal by Magnetic Separation-Based Blood Purification.

  • SNF P3 Forschungsdatenbank Project 131268

    Snf P3 Forschungsdatenbank Project 131268

    Jan 01, 2011 Endotoxin removal by magnetic separation-based blood purification Herrmann Inge K, Urner Martin, Graf Samuel, Schumacher Christoph M, Roth Zgraggen Birgit, Hasler Melanie, Stark Wendelin J, Beck-Schimmer Beatrice 2012, Endotoxin removal by magnetic separation-based blood purification, in Adv Health Mater , 26, 829-835.

  • Nanomedicine University Hospital Zurich

    Nanomedicine University Hospital Zurich

    Endotoxin removal by magnetic separation-based blood purification Adv Healthc Mater, 2012 26829-35. Bircher L, Theusinger OM, Locher S, Eugster P, Roth-Zgraggen B, Schumacher CM, Studt JD, Stark WJ, Beck-Schimmer B, Herrmann IK

  • Endotoxin reduction in nucleic acid purification Promega

    Endotoxin Reduction In Nucleic Acid Purification Promega

    Endotoxin Removal Capacity Assays with Silica Gel Particles and. Diatomaceous Earth . Two other silica-based materials were also tested for their capacity to remove endotoxins from a bacteria lysate solution under similar conditions to those used to remove endotoxins from the same type of solutions using magnetic silica particles in Examples ...

  • Journal Articles Nanoparticle Systems Engineering ETH

    Journal Articles Nanoparticle Systems Engineering Eth

    Endotoxin Removal by Magnetic Separation-Based Blood Purification. Advanced Healthcare Materials 2.6 2013 829-835. DOI 10.1002adhm.201200358 Research Collection

  • Magnetic particles for the separation and purification of

    Magnetic Particles For The Separation And Purification Of

    Oct 25, 2006 Nucleic acid separation is an increasingly important tool for molecular biology. Before modern technologies could be used, nucleic acid separation had been a time- and work-consuming process based on several extraction and centrifugation steps, often limited by small yields and low purities of the separation products, and not suited for automation and up-scaling.

  • A Biocompatible Method of Decorporation Bisphosphonate

    A Biocompatible Method Of Decorporation Bisphosphonate

    Endotoxin Removal by Magnetic Separation-Based Blood Purification. ... Nanomagnet-based removal of lead and digoxin from living rats. Nanoscale 2013, 5 18 ... Blood Purification Using Functionalized CoreShell Nanomagnets. Small 2010, 6 13 ...

  • Removal of Cells from Body Fluids by Magnetic Sepa

    Removal Of Cells From Body Fluids By Magnetic Sepa

    Magnetic separation offers a direct way for the removal of disease-causing factors from body fluids. Magnetic beads functionalized by a capturing moiety are employed to selectively bind to target com-pounds and are then subsequently removed by magnetic separation.1-3 A variety of beads, mostly based

  • Blood DNA Isolation Kit Magnetic Bead System Cat

    Blood Dna Isolation Kit Magnetic Bead System Cat

    Blood DNA Isolation Kit Magnetic Bead System Norgens Blood DNA Isolation Kit Magnetic Bead System is designed for the rapid preparation of genomic DNA from up to 200 L of whole blood from various species, including human. Purification is based on magnetic beads as the separation matrix. Norgens magnetic beads bind DNA under optimized ...

  • Christoph Schumacher Head of Development Powder

    Christoph Schumacher Head Of Development Powder

    However, both direct endotoxin detection in and removal from protein-rich fluids remains challenging. We present the synthesis and functionalization of ultra-magnetic cobaltiron alloy nanoparticles and a magnetic separation-based approach using polymyxin B-functionalized nanomagnets to remove endotoxin from human blood in vitro.

  • Endotoxin hitchhiking on polymer nanoparticles IOPscience

    Endotoxin Hitchhiking On Polymer Nanoparticles Iopscience

    Jun 02, 2016 Endotoxin is located in the bacterial cell membrane in the form of a complex lipopolysaccharide .When endotoxins come in contact with the body, it stimulates antigenic responses to gram-negative infections .The general symptoms of endotoxin exposure for humans include cough, fever, headache and nausea leading to multiple organ failures and the major manifestations of septic

  • Magnetic separation Find link

    Magnetic Separation Find Link

    Endotoxin removal by magnetic separation-based blood purification . Advanced Healthcare Materials. 2 6 Advanced Healthcare Materials. 2 6 Desulfovibrio magneticus 389 words view diff case mismatch in snippet view article find links to article

  • Endotoxin removal by magnetic separationbased blood

    Endotoxin Removal By Magnetic Separationbased Blood

    Abstract. This work describes a magnetic separation-based approach using polymyxin B-functionalized metal alloy nanomagnets for the rapid elimination of endotoxins from human blood in vitro and functional assays to evaluate the biological relevance of the blood purification process.

  • Hemocompatible magnetic particles with broadspectrum

    Hemocompatible Magnetic Particles With Broadspectrum

    It would contribute to the development of magnetic separation-based approaches to remove bacteria and bacterial endotoxin for extracorporeal blood purification, especially in initial sepsis therapy before pathogen identification.

  • Magnetic separationbased blood purification a

    Magnetic Separationbased Blood Purification A

    Review. Magnetic separation-based blood purification is especially attractive for the removal of high molecular weight compounds, which are poorly removed by conventional diffusion-based blood purifications systems e.g. dialysis, hemoadsorption Fig. 2 .The small size, the high surface-to-volume ratio and the high mobility of nanoparticles allow short diffusion distances and hence ...

  • Chitosan based in situ and ex situ magnetic iron oxide

    Chitosan Based In Situ And Ex Situ Magnetic Iron Oxide

    Fig. 4 a Schematic illustration of magnetic separation based protein purification using CHIO nanomaterials, b effect of chitosan loading on endotoxin removal by ex situ chitosan coated iron oxide nanoparticles Ex 5 1, Ex 10 1 amp Ex 20 1 and in situ iron oxide incorporated chitosan hydrogel nanocomposites In-3, c endotoxin removal ...

  • Inge K Herrmann Google Scholar

    Inge K Herrmann Google Scholar

    Endotoxin removal by magnetic separationbased blood purification IK Herrmann, M Urner, S Graf, CM Schumacher, B RothZgraggen, ... Advanced healthcare materials 2 6, 829-835 , 2013

  • Magnetic Blood Filtration as a Promising Treatment for

    Magnetic Blood Filtration As A Promising Treatment For

    Feb 12, 2021 Magnetic blood filtration technologies employ the powers of magnetism to selectively remove desired pathogenic substances from a patients blood. There are many approaches to implementing this technology, but one that has particularly attracted a lot of attention is the removal of pathogenic substances from the blood using targeted magnetic ...

  • Chromatographic Removal of Endotoxins A Bioprocess

    Chromatographic Removal Of Endotoxins A Bioprocess

    Gram-negative bacteria are widely used for the production of gene-based products such as DNA vaccines and bio-drugs, where endotoxin contamination can occur at any point within the process and its removal is of great concern. In this article, we review the structures of endotoxin and the effects that it causes in vivo . The endotoxin removal strategies are also discussed in the light of the ...

  • Biochemical functionality of magnetic particles as

    Biochemical Functionality Of Magnetic Particles As

    May 31, 2019 Herrmann IK, Urner M, Graf S, Schumacher CM, Roth-Zgraggen B, Hasler M, et al. Endotoxin removal by magnetic separation-based blood purification. Adv Healthc Mater. 2013282935. CAS PubMed Article Google Scholar 70.

  • US Patent for Magnetic capture of a target from a fluid

    Us Patent For Magnetic Capture Of A Target From A Fluid

    May 16, 2019 Disclosed herein is an improved method for magnetic capture of target molecules e.g., microbes in a fluid. Kits and solid substrates for carrying the method described herein are also provided. In some embodiments, the methods, kits, and solid substrates described herein are optimized for separation andor detection of microbes and microbe-associated molecular pattern MAMP

  • Device for continuous extracorporeal blood purification

    Device For Continuous Extracorporeal Blood Purification

    BACKGROUND The present work illustrates how magnetic separation-based blood purification using ultra-strong iron nanomagnets can be implemented into an extracorporeal blood purification circuit. By this promising technique, todays blood purification may be extended to specifically filter high-molecular compounds without being limited by ...

  • CA2329067C Endotoxin reduction in nucleic acid

    Ca2329067c Endotoxin Reduction In Nucleic Acid

    The prese nt method removes endotoxins from such solutions using silica-based materials, such as silica gel particles, magnetic silica particles, or diatomaceous earth. In a preferred aspect of the method of this invention, magnetic silic a particles are used to isolate plasmid DNA from a lysate of gram-negative bacteria transformed with the ...

  • Device for continuous extracorporeal blood purification

    Device For Continuous Extracorporeal Blood Purification

    Feb 10, 2011 A first prototype device of magnetic separation-based continuous extracorporeal blood purification was assembled from three parts i an injection port for nanomagnet administration into the bloodstream, ii a contact chamber where nanomagnets bind to the target molecules and iii a separator unit using magnetic field gradients to remove the ...

  • Nanomagnetbased removal of lead and digoxin from living

    Nanomagnetbased Removal Of Lead And Digoxin From Living

    Based on this new approach, current blood purification technologies can be extended to efficiently remove disease-causing factors, e.g. overdosed drugs, bacteria or cancer cells without being limited by filter cut-offs or column surface saturation.

  • WO1999054340A1 Endotoxin reduction in nucleic acid

    Wo1999054340a1 Endotoxin Reduction In Nucleic Acid

    The present invention presents a novel method for removing endotoxins from nucleic acids, such as DNA, RNA, or hybrids thereof, contaminated therewith. Nucleic acid solutions which can be treated using the method of this invention include, but are not limited to, lysates of gram-negative bacteria and nucleic acid solutions contaminated with endotoxins from external sources.

  • Recent advances in nanomedicine for sepsis treatment

    Recent Advances In Nanomedicine For Sepsis Treatment

    May 03, 2018 97 Herrmann IK, Urner M, Graf S et al. Endotoxin removal by magnetic separation-based blood purification. Adv. Healthc. Mater. 26, 829835 2013.Crossref, Medline, CAS, Google Scholar 98 Lee J-J, Jeong KJ, Hashimoto M et al. Synthetic ligand-coated magnetic nanoparticles for microfluidic bacterial separation from blood. Nano Lett. 141 ...

  • Specific Blood Purification by Means of Antibody

    Specific Blood Purification By Means Of Antibody

    By application in an in vitro MDS set-up, human plasma could be efficiently cleansed from IL-l and TNFa. Contrary to conventional blood and plasma chromatography techniques, MDS offers a high degree of flexibility and continuous blood processing due to magnetic adsorbent replacement.

  • Automated Purification of TransfectionGrade Plasmid

    Automated Purification Of Transfectiongrade Plasmid

    devices, such as the MagnaBot 96 Magnetic Separation Device, allowing walk-away automation of the procedure. The system builds upon our Wizard MagneSilTM Plasmid Purification System, but differs in two important aspects. It incorporates a novel mag-netic Endotoxin Removal Resin, used after the lysate clearing step,

  • A NOVEL MICROFLUIDIC CELLBASED BLOOD

    A Novel Microfluidic Cellbased Blood

    KEYWORDS Sepsis, Blood separation, Inflammation, Margination INTRODUCTION Sepsis represents an adverse systemic inflammatory response caused by microbial infection in blood 1. Current ex-tracorporeal blood purifications based on removal of cytokines and endotoxin

  • How nanotechnologyenabled concepts could contribute to

    How Nanotechnologyenabled Concepts Could Contribute To

    May 29, 2015 It has recently been shown that magnetic blood purification enables rapid removal of disease-causing factors from whole blood 2325. In magnetic blood purification, tiny magnetic particles functionalized with capturing ligands are injected into whole blood where they bind to target compounds Fig. 1. By applying an external magnetic ...

  • Automated Magnetic Separations for Proteomics Thermo

    Automated Magnetic Separations For Proteomics Thermo

    Magnetic particles comprise a convenient solid support for a variety of assays and procedures based on affinity purification. Paramagnetic beads are especially well suited for automated procedures because instrumentation exists to easily mix, incubate and separate the particles in 96-well plates without columns or centrifugation.

  • Endotoxin removal by magnetic separationbased blood

    Endotoxin Removal By Magnetic Separationbased Blood

    This work describes a magnetic separation-based approach using polymyxin B-functionalized metal alloy nanomagnets for the rapid elimination of endotoxins from human blood in vitro and functional assays to evaluate the biological relevance of the blood purification process.

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